Investigating multigenerational expression in the extremophile red alga Cyanidioschyzon merolae

The unicellular red algal Cyanidioschyzon merolae can conduct homologous recombination in its nuclear genome, however selection markers for this organism are limited. This limitation means that expressing multigenerational pathways in this host require a small set of transformation steps. This work will explore means by which to apply advanced synthetic biology techniques and designs to express multiple trans genes from single transformation events into the alga's nuclear genome

Systematically investigate various transformation and transgene design strategies in Cyanidioschyzon merolae.