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Development of a novel Stimulated Raman Scattering microscopy system

Project

Project Details

Program
BioScience
Field of Study
​Electrical Engineering, physics
Division
Biological and Environmental Sciences and Engineering
Faculty Lab Link
https://www.kaust.edu.sa/en/study/faculty/carlo-liberale

Project Description

Microscopy techniques based on vibrational spectroscopy are poised to be part of the next generation of microscopes for biological applications based on their unique chemical contrast and sub-cellular resolution for non-invasive, non-destructive and label free imaging of biological samples as live cells. The project will focus on the development of a fast and low-noise detection system in a setup for microscopic vibrational spectroscopy based on Stimulated Raman Scattering, which is one of the most advanced and sensitive methods for label-free microscopy for bio-imaging. The system will be applied to vibrational imaging of cancer stem cells to unveil their specific bio-chemical signatures. ​ ​​​​

About the Researcher

Carlo Liberale
Research Associate Professor, Bioscience
Biological and Environmental Science and Engineering Division

Affiliations

Education Profile

  • Ph.D., University of Pavia, Italy, 2004
  • M.Sc., University of Pavia, Italy, 2000

Research Interests

Prof. Liberale's research interests are focused on developing and applying label-free chemical imaging techniques based on vibrationalA spectroscopy (Infrared and Raman micro-spectroscopy) and multi-photon processes (Coherent Raman Microscopy, SHG). One of the mainA aims of this research activity is to unveil specific bio-chemical signatures of cancer stem cells, with a particular focus on understanding theA dysregulation of their lipid metabolism. He is also interested on using high-resolution 3D printing based on Direct Laser Writing for the fabrication of novel micro-optics, towardsA the miniaturization of complex optical systems, and of smart micro-/nano-structures to be used as a probe in nanoscale imaging. ThisA research activity takes advantage from an integrated approach that combines design, micro/nanofabrication and optical techniques.a€‹

Selected Publications

  • Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness A Antonini, A Sattin, M Moroni, S Bovetti, C Moretti, F Succol, A Forli, D Vecchia, V P Rajamanickam, A Bertoncini, S Panzeri, C Liberale, T Fellin, eLife 9:e58882 (2020)
  • 3D printed waveguides based on Photonic Crystal Fiber designs for complex fiber-end photonic devices, A Bertoncini, C Liberale, Optica 7 (11), 1487-1494 (2020)
  • Fingerprinta€toa€CH stretch continuously tunable high spectral resolution Stimulated Raman Scattering microscope, S P Laptenok, V P Rajamanickam, L Genchi, T Monfort , Y Lee, I I Patel, A Bertoncini, C Liberale, Journal of Biophotonics 12(9) e201900028 (2019)
  • Lipid droplets: a new player in colorectal cancer stem cells unveiled by spectroscopic imaging, L Tirinato, C Liberale, S Di Franco, P Candeloro, A Benfante, R La Rocca, L Potze, R Marotta, R Ruffilli, V P Rajamanickam, M Malerba, F De Angelis, A Falqui, E Carbone, M Todaro, J Medema, G Stassi, E Di Fabrizio, Stem Cells 33 (1), 35-44 (2015)
  • Miniaturized all-fibre probe for three-dimensional optical trapping and manipulation, C Liberale, P Minzioni, F Bragheri, F De Angelis, E Di Fabrizio, I Cristiani, Nature Photonics 1 (12), 723-727 (2007)

Desired Project Deliverables

​Learn Coherent Raman Scattering techniques. Design, assemble and test circuitry for multiplexed and low-noise detection in a Stimulated Raman Scattering microscopy setup based on femtosecond broadband laser sources. Demonstrate fast and high S/N ratio imaging with multiplex (broadband) Stimulated Raman Scattering microscopy. ​ 
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