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Microbes for coral reef recovery: exploring bacterial cues for coral settlement

Project

Project Details

Program
Marine Science
Field of Study
Marine biology, Coral reef ecology, Microbiology, Coral restoration
Division
Biological and Environmental Sciences and Engineering
Center Affiliation
Red Sea Research Platform

Project Description

Coral reefs are among the most diverse and valuable ecosystems on Earth, yet they are declining worldwide due to climate change and other human impacts. The long-term persistence of coral reefs depends on the successful settlement and recruitment of new corals. Coral larvae do not settle randomly; instead, they rely on specific environmental cues that signal suitable habitat for growth and survival. Crustose coralline algae (CCA), a group of calcifying red algae common on healthy reefs, are known to promote coral settlement. Increasing evidence suggests that bacteria associated with the surface of these algae play a critical role in producing the chemical signals that induce coral larvae to settle and metamorphose. The goal of this project is to contribute to ongoing coral restoration research at KAUST by identifying bacterial strains associated with Red Sea CCA that promote coral settlement and characterizing the chemical compounds responsible for this activity. Students will contribute to the isolation and cultivation of CCA-associated bacteria, molecular characterization of bacterial communities, preparation of bacterial biofilms, and extraction of bacterial metabolites. Promising bacterial strains and their chemical extracts will be tested in coral settlement experiments, with the long-term goal of developing tools that can enhance coral recruitment and support reef restoration efforts. This work is part of a funded research program within the Global Change Ecology Lab, under the supervision of Dr. Aurora Giorgi and Professor Maggie Johnson, and is conducted in collaboration with the KAUST Coral Restoration Initiative (KCRI).

About the Researcher

Maggie Johnson
Assistant Professor, Marine Science
Biological and Environmental Science and Engineering Division

Education Profile

  • Postdoctoral Scholar, Woods Hole Oceanographic Institution, 2019-2021
  • Postdoctoral Fellow, Smithsonian Institution, 2016-2019
  • Ph.D. in Marine Biology, Scripps Institution of Oceanography, 2011-2016
  • M.S. in Biology, California State University, Northridge, 2008-2011
  • Prof. M.S. in Marine Biology, Northeastern University Three Seas Program, 2005-2007
  • B.A. in Biology, Colby College 2001-2005

Research Interests

Professor Johnson's research interests are centered on the impacts of environmental change on coral reef ecosystems. She uses a combination of field and laboratory based approaches to 1) evaluate and monitor the structure and function of coral reef ecosystems, 2) quantify inherent natural heterogeneity in key environmental parameters including temperature, dissolved oxygen, and pH , 3) explore the implications of environmental variability for ecophysiology of coral reef primary producers, 4) and determine the implications of local and global environmental change for these foundational coral reef taxa. Professor Johnson's research includes taxa ranging from fleshy algae to corals, but she primarily focuses on calcifying algae - particularly crustose coralline algae. She uses calcifying reef algae as model taxa to identify and monitor coral reef ecosystem responses in an era of rapid environmental change.

Selected Publications

  • Johnson MD, JS Scott, M Leray, N Lucey, LM Rodriguez Bravo, W Wied, AH Altieri (2021) Rapid ecosystem-scale consequences of acute deoxygenation on a Caribbean coral reef. Nature Communications. 12(4522). DOI 10.1038/s41467-021-24777-3.
  • Johnson MD, SD Swaminathan, EN Nixon, V Paul, AH Altieri. (2021) Differential susceptibility of reef-building corals to deoxygenation reveals remarkable hypoxia tolerance. Scientific Reports.
  • Johnson MD, MD Fox, ELA Kelly, B Zgliczynski, Stuart A Sandin, JE Smith (2020) Ecophysiology of coral reef primary producers across an upwelling gradient in the tropical central Pacific. PLOSOne. 15(2): e0228448.
  • Johnson MD, RC Carpenter (2018) Nitrogen enrichment offsets the direct negative effects of ocean acidification on a reef-building crustose coralline alga. Biology Letters DOI 10.1098/rsbl.2018.0371.
  • Johnson MD, S Comeau, C Lantz, JE Smith (2017) Complex and interactive effects of ocean acidification and temperature on epilithic and endolithic coral reef turf algal assemblages. Coral Reefs DOI 10.1007/s00338-017-1597-2.

Desired Project Deliverables

Depending on the student's skills and project duration, deliverables may include: · Isolation and culturing of bacteria associated with crustose coralline algae. · Preparation of microbiology media · Daily and weekly maintenance of bacterial cultures · Development of monospecific bacterial biofilms for settlement assays. · Extraction and fractionation of bacterial metabolites. · Analysis of coral larval settlement experiment results.

Recommended Student Background

Marine science
Microbiology
Environmental science
Ecology
Chemical biology/ecology