Identification and Characterization of Target-Specific Nanobodies Using Display Selection Platforms
Project Details
Project Description
Display technologies, such as phage display, bacterial display, cell surface display, and mRNA display, have revolutionized the field of antibody engineering and therapeutic antibody discovery. These technologies enable the identification of high-affinity, specific binders for various targets. Nanobodies, the smallest functional antibody fragments derived from camelid heavy-chain-only antibodies, are ideal for diagnostics, therapeutics, and biosensing application due to their small size, stability, and solubility. This project focuses on the high-throughput screening of nanobodies against novel diagnostic and therapeutic targets using display-based selection.
Project-duration will be 3-6 month, details of arrival/departure dates to be discussed.
About the Researcher
Affiliations
Education Profile
- Postdoctoral fellow, Harvard University
- Ph.D., ETH Zurich
- B.Sc./M.Sc., Technical University of Berlin
Research Interests
Professor Rueping's research interests include organocatalysis, metal catalysis and biocatalysis; new reaction methodology and technology; smart materials, photochemistry and flow chemistry.Selected Publications
- H.-H. Liao, A. Chatupheeraphat, C.-C. Hsiao, I. Atodiresei, M. Rueping. ""Asymmetric BrA¸nsted Acid Catalyzed Synthesis of Triarylmethanes - Construction of Communesin- and Spiroindoline Scaffolds."" Angew. Chem. Int. Ed. 54, 15540-15544
- M. Nakajima, E. Fava, S. Loescher, Z. Jiang, M. Rueping. ""Photoredox-Catalyzed Reductive Coupling of Aldehydes, Ketones, and Imines with Visible Light."" Angew. Chem. Int. Ed. 54, 8828-8832
- D. C. Fabry, M. A. Ronge, J. Zoller, M. Rueping. ""C-H Functionalization of Phenols Using Combined Ruthenium and Photoredox Catalysis: In Situ Generation of the Oxidant."" Angew. Chem. Int. Ed. 54, 2801-2805
- S. Raja, M. Nakajima, I. Atodiresei, M. Rueping. ""Combined Experimental and Computational Study for the First Catalytic Asymmetric 4p-Elektrocyclization of N-Heterocycles."" Angew. Chem. Int. Ed. 54, 2762-2765
- M. Leiendecker, C.-C. Hsiao, L. Guo, N. Alandini, M. Rueping. ""Metal-Catalyzed Dealkoxylative Caryl-Csp3 Cross-Coupling - Replacement of Aromatic Methoxy Groups Employing a Functionalized Nucleophile"" Angew. Chem. Int. Ed. 53, 12912-12915
Desired Project Deliverables
Project-duration will be 3-6 month, details of arrival/departure dates to be discussed.
Students shall extend their general knowledge and skills in molecular biology and protein biochemistry. An emphasis will be put on the use of display selection platforms, and protein expression, interaction and characterization techniques. Students will be taught to work independently on projects, yet strengthening their critical sense to develop new ideas. In the course of the internship students shall demonstrate this understanding during oral presentations and one final written report.
A background in biochemistry is preferred, but students with backgrounds in chemistry, biotechnology or biology are invited to apply.
The ideal candidates should have some experience or interest in conducting quantitative library screening through fluorescent-activated cell sorting (FACS).
Experience with or interest in learning laboratory skills associated with display library screening is beneficial.