Developing molecular method FISH to visualize RNA-condensates in desert plants
Project Details
Program
Plant Science
Field of Study
Cell Biology, Crop improvement, Molecular Biology
Division
Biological and Environmental Sciences and Engineering
Faculty Lab Link
Project Description
Working with desert plants, that are not transformable is very challenging. We have already tools existing in the lab that can be adjusted for desert plant to monitor stress response at the cellular level. Candidate will learn how to culture different plant species in laboratory conditions and optimize technique for RNA-condensate visualization to be able to understand cellular stress response in those different desert plants. Therefore, candidate will learn how to work with agronomically important plants, how to use molecular biology tools and how to study cellular processes using confocal microscopy.
About the Researcher
Monika Chodasiewicz
Assistant Professor, Plant Science
Affiliations
Education Profile
- Postdoctoral Fellow, Biochemistry, Max Planck Institute of Molecular Plant Physiology, Germany 2014-2020
- PhD, Plant Molecular Physiology, Max Planck Institute of Molecular Plant Physiology, Germany 2010-2014
- B.Sc and M.S., Biotechnology, University of Szczecin, Poland 2005-2010
Research Interests
Professor Chodasiewicz' s research focuses on identification of stress specific components (proteins, metabolites and mRNA) of stress granules (SGs) formed under different environmental conditions. Professor Chodasiewicz's research group also aims to understand what are the stress specific components of desert plants which make them resistant to stressful environmental conditions. This research line involves studying small molecule-protein complexes with biochemical methods combined with molecular biology. The team intends to build a map of stress specific small molecule-protein complexes to further dissect the mode of action of stress specific complexes with molecular biology and cell biology methods.Selected Publications
- Protein and metabolite composition of Arabidopsis stress granules, Kosmacz M., Gorka M., Schmidt S., Luzarowski M., Moreno J.C., Szlachetko J., Leniak E., Sokolowska E.M., Sofroni K., Schnittger A., Skirycz A., New Phytologist, 2019.
- Interaction of 2',3'-cAMP with Rbp47b plays a role in stress granule formation, Kosmacz M., Luzarowski M., Kerber O., Leniak E., Gutiérrez-Beltrán E., Moreno J.C., Gorka M., Szlachetko J., Veyel D., Graf A., Skirycz A., Plant Physiology, 2018.
- PROMIS, global analysis of Protein–Metabolite Interactions using size separation in Arabidopsis thaliana, Veyel D., Sokolowska E., Moreno J.C., Kierszniowska S., Cichon J., Wojciechowska I., Luzarowski M., Kosmacz M., Szlachetko J., Méret M., Graf A., Meyer E., Willmitzer L., Skirycz A., Journal of Biological Chemistry, 2018.
- The stability and nuclear localization of the transcription factor RAP2.12 are dynamically regulated by oxygen concentration, Kosmacz M., Parlanti S., Schwarzlander M., Kragler F., Licausi F., van Dongen J.T., Plant Cell and Environment, 2014.
- Oxygen sensing in plants is mediated by an N-end rule pathway for protein destabilization, Licausi F., Kosmacz M., Weits D., Giuntoli B., Giorgi F.M., Voesenek L.A.C.J., Perata P., and van Dongen J.T., Nature, 2011, 479, 419422.
Desired Project Deliverables
-establish culturing system for 5 different plant species (with different varieties - sensitive and tolerant to different stresses)
- establish heat stress, salinity and low oxygen stress treatments for those desert plants
- establish FISH-RNA method for microscopical visualization of cellular condensates in the root cells.
Recommended Student Background
basic knowledge on cell and biochemical processes
precision in the lab
motivated and responsisble