An iPSCs-based approach to model Type Two Diabetes in-vitro
Project Details
Program
BioScience
Field of Study
Molecular and Cellular Biology and/or Bioinformatics
Division
Biological and Environmental Sciences and Engineering
Faculty Lab Link
Project Description
Studying the transcriptional and epigenetic mechanisms dysregulated in patients affected by metabolic disorders such as insulin resistance (IR) and type 2 diabetes mellitus (T2DM) is essential to derive efficient pharmacological approaches. We are seeking an outstanding student to work on a project focused on the study of the role of histone modifiers to the onset of metabolic disorders.
About the Researcher
Antonio Adamo
Associate Professor, Bioscience
Affiliations
Education Profile
- FEBS Senior Postdoctoral Fellow, European Institute of Oncology (IEO), Italy, 2012-2015
- Postdoctoral Fellow, Center of Regenerative Medicine of Barcelona (CMRB), Spain, 2009-2012
- Ph.D. in Biotechnologies applied to Medical Sciences, University of Milan, 2008
- M.Sc., Medical Biotechnologies and Molecular Medicine, University of Milan, 2005
- B.Sc., Medical Biotechnologies, University of Milan, 2003
Research Interests
Professor Adamo's research interests focus on the use of human embryonic stem cells (hESCs) and patient-derived induced pluripotent stem cells (iPSCs) to model the onset and progression of human disorders linked to copy number variations a€œin a dish.a€ His team developed the largest cohort of Klinefelter syndrome (karyotype 47,XXY) and high-grade X aneuploid iPSCs (karyotype 48,XXXY and 49,XXXXY) that he uses to study the molecular dysregulations associated with X chromosome aneuploidy during the earliest stages of human development. Professor Adamoa's team combines reprogramming, organoid derivation, and genome editing techniques with a multi-omics approach to identify the transcriptional and epigenetic signatures underlying human diseases.Selected Publications
- Astro V, Alowaysi M, Fiacco E, Saera-Vila A, Cardona-LondonIƒo KJ, Aiese Cigliano R, Adamo A: Pseudoautosomal Region 1 Overdosage Affects the Global Transcriptome in iPSCs From Patients With Klinefelter Syndrome and High-Grade X Chromosome Aneuploidies. Frontiers in Cell and Developmental Biology 2021.
- Astro V., Ramirez-Calderon G., Pennucci R., Caroli J., Saera-Vila A., Cardona-London Iƒo K., Forastieri C., Fiacco E., Maksoud F., Alowaysi M., Sogne E., Falqui A., Gonzalez F., Montserrat N., Battaglioli E., Mattevi A., and Adamo A. Fine-tuned KDM1A alternative splicing regulates human cardiomyogenesis through an enzymatic-independent mechanism. iScience 25, July 15, 2022.
- Fiacco E, Alowaysi M, Astro V, Adamo A: Derivation of two naturally isogenic iPSC lines (KAUSTi006-A and KAUSTi006-B) from a mosaic Klinefelter Syndrome patient (47-XXY/46-XY). Stem Cell Research Volume 49 December 2020.
- Alowaysi M, Astro V, Fiacco E, Adamo A: Generation of two iPSC lines (KAUSTi001-A, KAUSTi002-A) from a rare high-grade Klinefelter Syndrome patient (49-XXXXY) carrying a balanced translocation t(4,11) (q35,q23). Stem Cell Research Volume 49 December 2020.
- Adamo A., Atashpaz S., Germain P.-L., Zanella M., Da'Agostino G., Albertin V., Chenoweth J., Micale L., Fusco M., Unger C., Augello B., Palumbo O., Hamilton B., Carella M., Donti E., Pruneri G., Selicorni A., Biamino E., Prontera P., McKay R., Merla G. & Testa G. (2015) 7q11.23 dosage-dependent dysregulation in human pluripotent stem cells affects transcriptional programs in disease-relevant lineages. Nature Genetics 2015 Feb;47(2):132-41
- Adamo A, Barrero MJ, IzpisAºa Belmonte JC. (2011) LSD1 and pluripotency: a new player in the network. Cell Cycle 10(19): 3215-6.
- Adamo A, SesA© B, Boue S, CastaA±o J, Paramonov I, Barrero MJ, IzpisAºa Belmonte JC. (2011) LSD1 regulates the balance between self-renewal and differentiation in human embryonic stem cells. Nature Cell Biology 13(6): 652-60.
Desired Project Deliverables
The selected candidate will use human stem cells and terminally differentiated glucose sensitive cell types and will acquire skills in molecular biology techniques including Chromatin Immuno-precipitation (ChIP), quantitative real-time PCR (Q-PCR) and next generation sequencing (NGS).